HarvESTer™ Analysis System

The HarvESTer™ Analysis System is a sophisticated, high-throughput clustering and assembly tool, offering a series of flexible modules designed for incrementally added EST data processing. The system is used in industrial-scale projects at commercial partners where its flexibility is ideal for creating customized schemes to meet project-specific requirements. A powerful database facilitates high-throughput analysis while intuitive and interactive viewers facilitate manual configuration, interaction and analysis of results.

"In collaboration with KWS SAAT AG, Biomax has greatly expanded the HarvESTer software, a proven multiple-sequence assembly tool.

  • Two new modules, the Genotype Detector and the SNP Detector, were developed. In comparative sequencing, these modules enable semi-automatic detection of SNPs and indels in different genotypes, even if they are heterozygotes.
  • Special features allow the data to be exported in database-specific formats for further processing.
  • Previously processed and assembled sequences can be imported, edited, analyzed and combined with additional data for further analysis, including incremental processing.
  • Flexible import formats facilitate the combination and analysis of data from numerous project partners.

Overall, the HarvESTer tool is an integral part of the high-throughput analysis pipeline for the development of diagnostic, molecular markers at KWS; it minimizes manual labor, reduces completion time and improves the efficiency of the entire analysis process."

— Dr. Andreas Menze, RD Information Management, KWS SAAT AG, Germany

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The HarvESTer system allows the following:

  • Use of quality and trace files
  • Configuration of custom workflows
  • Annotation and editing of sequences, clusters and assemblies
  • Performance of clone linking
  • Detection of SNPs
  • Differentiation between homozygote and heterozygote